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Slide 004

Slide ID: 004
Collection of murine pulmonary tumors - Slide 004
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Submitting Investigator: R. Ilona Linnoila
Mouse Age: 1.5 month
Mouse Sex: Female
Mouse Strain: B6D2F1xlCR
Genotype: TgCC10-SV40Tag, TgCC10hASH1 (bitransgenic)
Genetic Modification Details: Mouse CC10-hASH1 transgene was cloned by fusing 1.1 kb of sequence containing the coding region of the hASH1 (human achaete homologue-1) gene from pM1.1 with 2.1kb of upstream 5'-flanking sequences of the mCC10 (mouse Clara cell specific10kD protein) promoter. Doubly transgenic animals obtained by: CC10-hASH x CC10-SV40 Tag (strain7736) mice.
Other Factors: NA
Gross Morphology: Enlarged lung. Body weight 24.1g at sacrifice
Method of Fixation: NBF
Annotations: Adenocarcinoma with neuroendocrine features.
There are five well demarcated small neoplastic areas, centered on terminal bronchioles that are lined by hyperplastic epithelium with short papillary projections. The neoplastic cells form irregular tubular-papillary undulating arrays with crowded lining epithelial cells piling up on top of one other. The cells are short columnar with oval dark eccentric (basal) nuclei and scant cytoplasm. There are many mitotic figures (80 in 10 HPF). There are occasional individual cell death mixed with necrotic debris within some of the lumens. The rest of the pulmonary parenchyma is normal.
References:

Linnoila, R. I., Sahu, A., Miki, M., Ball, D. W., and DeMayo, F. J. (2000). Morphometric analysis of CC10-hASH1 transgenic mouse lung: a model for bronchiolization of alveoli and neuroendocrine carcinoma. Exp. Lung. Res. 26, 595-615.

Linnoila, R. I., Zhao, B., DeMayo, J. L., Nelkin, B. D., Baylin, S. B., DeMayo, F. J., and Ball, D. W. (2000). Constitutive achaete-scute homologue-1 promotes airway dysplasia and lung neuroendocrine tumors in transgenic mice. Cancer Res. 60, 4005-4009.