conditional knockout alleles for both p53 and Rb. Both genes become
full null alleles upon Cre-mediated recombination.
has been intratracheally infected with AdCre and sacrificed at 30
weeks post infection.
did contain macroscopic tumor lesions.
Method of Fixation:
There is a densely cellular closely arranged neoplastic cell population
that effaces the normal pulmonary architecture. The neoplastic population
is arranged in a solid patternwith poorly defined nests and
streams. The neoplastic cells are small to medium size and
round with scant cytoplasm, finely granular chromatin and small nucleoli.
The neoplastic cells are supported by a fine fibrovascular stroma. Adjacent
compressed alveolar spaces contain small aggregates of neoplastic cells
and swollen macrophages. Small bronchioles are lined by prominent
hyperplastic robust epithelium and the lumen contain fibrin mixed with
small neoplastic aggregates. There are occasional aggregates
that resemble blood vessels filled with neoplastic cells and mixed with
erythrocytes. There are small areas of necrosis scattered
throughout the tumor. According to immunohistochemistry,
the tumor cells contain variable amounts of synaptophysin and NSE, but
not CD3 and CD45R antigens.
Meuwissen, R., Linn,
S. C., Linnoila, R. I., Zevenhoven, J., Mooi, W. J., and Berns, A. Induction of small cell lung
cancer by somatic inactivation of both Trp53 and Rb1 in a conditional mouse model. Cancer Cell,
4: 181-189, 2003.