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The components of the immune system have diverse roles in the initial development of cancers, progression of early-stage malignancies to invasive tumors, establishment of metastatic lesions, tumor dormancy, and response or resistance to therapy.  Characterizing the components of the immune system and their functional status in tissues and in tumors requires the use of highly specific reagents.  Researchers employ antibodies in a variety of in vitro and in vivo applications to delineate, enrich, or deplete specific immune subsets in order to understand their role(s) in tumorigenesis. 

 

This is a glossary of validated reagents and protocols that are useful for functional phenotyping of the immune system in murine cancer models.

 

anti-bacterial GST

Antibody against:anti-bacterial GST
Label:
Clone: PIP
Isotype: Armenain hamster Ig
Type: purified hybridoma supernatant
Source:
Used for: control Ig
Treatment / Staining Regime: control Ig
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-BrdU

Antibody against:anti-BrdU
Label: FITC
Clone: 3D4
Isotype: Mouse IgG1, k
Type: commercial
Source: Pharmingen 51-33284X
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-GFP

Antibody against:anti-GFP
Label: AlexaFlour 647
Clone: FM264G
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 338005
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-Hamster IgG

Antibody against:anti-Hamster IgG
Type: Secondary Antibody
Label: PE
Clone: Poly4055
Isotype: Goat
Type: commerciai
Source: BioLegend 405506
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-human CD1b

Antibody against:anti-human CD1b
Label: FITC
Clone: M-T101
Isotype: Mouse IgG1, k
Type: commercial
Source: Pharmingen 555969
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-human CD1b

Antibody against:anti-human CD1b
Label: none
Clone: M-T101
Isotype: Mouse IgG1, k
Type: commercial
Source: BD Pharmingen 555968
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-human IFNGRalpha / CD119

Antibody against:anti-human IFNGRalpha / CD119
Label: none
Clone: GIR-208
Isotype: Mouse IgG1, k
Type: purified hybridoma supernatant
Source:
Used for: control Ig
Treatment / Staining Regime: isotype control - match dose
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-human IL-10R Beta

Antibody against:anti-human IL-10R Beta
Label: Biotin
Clone:
Isotype: goat IgG
Type: commercial
Source: R and D systems BAF874
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD16/32

Antibody against:anti-mouse CD16/32
Label: Biotin
Clone: 2.4G2
Isotype: Rat IgG2b, k
Type: commercial
Source: Pharmingen 01242D, new = 553143
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse 34

Antibody against:anti-mouse 34
Label: FITC
Clone: RAM34
Isotype: Rat IgG2a, k
Type: commercial
Source: Pharmingen 553733
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD1d

Antibody against:anti-mouse CD1d
Label: none
Clone: 1B1
Isotype: Rat IgG2b, k
Type: commercial
Source: Pharmingen 09901D, new = 553843
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD1d

Antibody against:anti-mouse CD1d
Label: none (no azide / low endotoxin)
Clone: 1B1
Isotype: Rat IgG2b, k
Type: commercial
Source: Pharmingen 559438
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD1d

Antibody against:anti-mouse CD1d
Label: PE
Clone: 1B1
Isotype: Rat IgG2b, k
Type: commercial
Source: BD Pharmingen 553846
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD1d

Antibody against:anti-mouse CD1d
Label: FITC
Clone: 1B1
Isotype: Rat IgG2b, k
Type: commercial
Source: Pharmingen 553845
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD2 / LFA

Antibody against:anti-mouse CD2 / LFA
Label: Biotin
Clone: RM2-5
Isotype: Rat IgG2b, lambda
Type: commercial
Source: Pharmingen 01172D, new = 553110
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD3e

Antibody against:anti-mouse CD3e
Label: PE
Clone: 145-2C11
Isotype: Hamster IgG
Type: commercial
Source: BioLegend 100308
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD3e

Antibody against:anti-mouse CD3e
Label: FITC
Clone: 145-2C11
Isotype: Hamnster IgG
Type: commercial
Source: BioLegend 100306
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD3e

Antibody against:anti-mouse CD3e
Label: APC
Clone: 145-2C11
Isotype: Hamster IgG
Type: commercial
Source: BioLegend 100312
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD4

Antibody against:anti-mouse CD4
Label: APC
Clone: GK1.5
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 100412
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD4

Antibody against:anti-mouse CD4
Label: none
Clone: GK1.5
Isotype: Rat IgG2b, k
Type: purified hybridoma supernatant
Source:
Used for: depletion in vivo
Treatment / Staining Regime: 750 ug i.p. on day -1, and 250 ug weekly maintenance dose.
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD4

Antibody against:anti-mouse CD4
Label: PE
Clone: RM4-5
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 100512
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD4

Antibody against:anti-mouse CD4
Label: FITC
Clone: GK1.5
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 100406
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD4

Antibody against:anti-mouse CD4
Label: PE
Clone: CT-CD4
Isotype: Rat IgG2a
Type: commercial
Source: Caltag RM2504
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD5

Antibody against:anti-mouse CD5
Label: none
Clone: 53-7.3
Isotype: Rat IgG2a, k
Type: commercial
Source: Pharmingen 01031D, new = 553017
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD8a

Antibody against:anti-mouese CD8a
Label: PE
Clone: 53-6.7
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 100708
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD8a

Antibody against:anti-mouse CD8a
Label: CyChrome
Clone: 53-6.7
Isotype: Rat IgG2a, k
Type: commercial
Source: Pharmingen 553034
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD8a

Antibody against:anti-mouse CD8a
Label: none
Clone: YTS-169.4
Isotype: Rat IgG2b
Type: purified hybridoma supernatant
Source:
Used for: depletion in vivo
Treatment / Staining Regime: 750 ug i.p. on day -1, and 250 ug weekly maintenance dose.
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse cD8a

Antibody against:anti-mouse cD8a
Label: FITC
Clone: 53-6.7
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 100706
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD8a

Antibody against:anti-mouse CD8a
Label: APC
Clone: 53-6.7
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 100712
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD8a

Antibody against:anti-mouse CD8a
Label: tri-color
Clone: ?
Isotype: ?
Type: commercial
Source: Caltag RM2206
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD8B

Antibody against:anti-mouse CD8B
Label: PE
Clone: 53-5.8
Isotype: Rat IgG1, k
Type: commercial
Source: BioLegend 140407
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD8B

Antibody against:anti-mouse CD8B
Label: PerCP-Cy5.5
Clone: YTS156.7.7
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 126610
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD11b

Antibody against:anti-mouse CD11b
Label: Biotin
Clone: M1/70
Isotype: Rat IgG2b, k
Type: commercial
Source: Pharmingen 553309
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD11b

Antibody against:anti-mouse CD11b
Label: PE
Clone: M1/70
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 101208
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD11b

Antibody against:anti-mouse CD11b
Label: FITC
Clone: M1/70
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 101206
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD11b

Antibody against:anti-mouse CD11b
Label: APC
Clone: M1/70
Isotype: Rat IgG2b, k
Type: comnercial
Source: BioLegend 101212
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD11c

Antibody against:anti-mouse CD11c
Label: PE
Clone: HL3
Isotype: Hamster IgG1
Type: commercial
Source: BD Pharmingen 553802
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD11c

Antibody against:anti-mouse CD11c
Label: FITC
Clone: N418
Isotype: Hamster IgG
Type: commercial
Source: BioLegend 117306
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD11c

Antibody against:anti-mouse CD11c
Label: APC
Clone: N418
Isotype: Hamster IgG
Type: commercial
Source: BioLegend 117310
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD11c

Antibody against:anti-mouse CD11c
Label: Biotin
Clone: N418
Isotype: Hamster IgG
Type: commercial
Source: BioLegend 117304
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD11c

Antibody against:anti-mouse CD11c
Label: APC
Clone: HL3
Isotype: Hamster IgG1
Type: commercial
Source: Pharmingen 550261
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD14

Antibody against:anti-mouse CD14
Label: PerCP-Cy5.5
Clone: Sa14-2
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 123314
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD14

Antibody against:anti-mouse CD14
Label: PE
Clone: Sa14-2
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 123309
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD16/32

Antibody against:anti-mouse CD16/32
Label: PE
Clone: 93
Isotype: Rat IgG2a, l
Type: commercial
Source: BioLegend 101307
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD16/32

Antibody against:anti-mouse CD16/32
Label: none
Clone: 2.4G2
Isotype: Rat IgG2b, k
Type: commercial
Source: BD Pharmingen 553142
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD24

Antibody against:anti-mouse CD24
Label: FITC
Clone: M1/69
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 101805
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD24

Antibody against:anti-mouse CD24
Label: PE/Cy7
Clone: M1/69
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 101821
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD24

Antibody against:anti-mouse CD24
Label: PerCP-Cy5.5
Clone: M1/69
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 101823
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD24 / HAS

Antibody against:anti-mouse CD24 / HAS
Label: Biotin
Clone: M1/69
Isotype: Rat IgG2b, k
Type: commercial
Source: Pharmingen 01572D, new= 553260
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD24 / HSA

Antibody against:anti-mouse CD24 / HSA
Label: Biotin
Clone: 30-F1
Isotype: Rat IgG2c, k
Type: commercial
Source: Pharmingen 555296
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD25

Antibody against:anti-mouse CD25
Label: PE
Clone: PC61
Isotype: Rat IgG1, k
Type: commercial
Source: BioLegend 102008
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD25

Antibody against:anti-mouse CD25
Label: PE
Clone: 7D4
Isotype: Rat IgM, k
Type: commercial
Source: BD Pharmingen 558642
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD31

Antibody against:anti-mouse CD31
Label: PE
Clone: MEC13.3
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 102508
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD40

Antibody against:anti-mouse CD40
Label: FITC
Clone: HM40-3
Isotype: Hamster IgM, k
Type: commercial
Source: Pharmingen 09404D
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD40

Antibody against:anti-mouse CD40
Label: PE
Clone: 1C10
Isotype: Rat IgG2a
Type: commercial
Source: BioLegend 102806
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD43

Antibody against:anti-mouse CD43
Label: FITC
Clone: S7
Isotype: Rat IgG2a, k
Type: commercial
Source: Pharmingen 01604D
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD43

Antibody against:anti-mouse CD43
Label: PE
Clone: S7
Isotype: Rat IgG2a, k
Type: commercial
Source: Pharmingen 01605B
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD44

Antibody against:anti-mouse CD44
Label: FITC
Clone: IM7
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 103006
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD44

Antibody against:anti-mouse CD44
Label: PE
Clone: IM7
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 103008
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD44

Antibody against:anti-mouse CD44
Label: APC
Clone: IM7
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 103012
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD45

Antibody against:anti-mouse CD45
Label: PE
Clone: 30-F11
Isotype: Rat IgG2b,k
Type: commercial
Source: BioLegend 102105
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD45 / LCA

Antibody against:anti-mouse CD45 / LCA
Label: CyChrome
Clone: 30-F11
Isotype: Rat IgG2b, k
Type: commercial
Source: Pharmingen 01118A, new = 553082
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD45.2

Antibody against:anti-mouse CD45.2
Label: FITC
Clone: 104
Isotype: Mouse IgG2a, k
Type: commercial
Source: BioLegend 109806
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD45.2

Antibody against:anti-mouse CD45.2
Label: PE
Clone: 104
Isotype: Mouse IgG2a, k
Type: commercial
Source: BioLegend 109808
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD45R/B220

Antibody against:anti-mouse CD45R/B220
Label: PE
Clone: RA3-6B2
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 103208
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD45R/B220

Antibody against:anti-mouse CD45R/B220
Label: PE/Cy5
Clone: RA3-6B2
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 103210
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD45R/B220

Antibody against:anti-mouse CD45R/B220
Label: none
Clone: RA3-6B2
Isotype: Rat IgG2a, k
Type: commercial
Source: Pharmingen 01121D, new = 553084
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD45R/B220

Antibody against:anti-mouse CD45R/B220
Label: FITC
Clone: RA3-6B2
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 103206
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD45RA

Antibody against:anti-mouse CD45RA
Label: Biotin
Clone: 14.8
Isotype: Rat IgG2b, k
Type: commercial
Source: Pharmingen 557460
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD45RB

Antibody against:anti-mouse CD45RB
Label: PE
Clone: C363-16A
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 103307
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD49b

Antibody against:anti-mouse CD49b
Label: FITC
Clone: DX5
Isotype: Rat IgM, k
Type: commercial
Source: Pharmingen 553857
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD49b

Antibody against:anti-mouse CD49b
Label: PE
Clone: DX5
Isotype: Rat IgM, k
Type: commercial
Source: BioLegend 108908
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD49b

Antibody against:anti-mouse CD49b
Label: APC
Clone: DX5
Isotype: Rat IgM, k
Type: commercial
Source: BioLegend 108910
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD49f

Antibody against:anti-mouse CD49f
Label: Biotin
Clone: GoH3
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 313604
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD51 / integrin alpha v

Antibody against:anti-mouse CD51 / integrin alpha v
Label: Biotin
Clone: H9.2B8
Isotype: Hamster IgG3, lambda
Type: commercial
Source: Pharmingen 557408, old = 01522A (discontinued)
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD54 / ICAM-1

Antibody against:anti-mouse CD54 / ICAM-1
Label: none
Clone: clone 3E2
Isotype: Hamster IgG1, k
Type: commercial
Source: Pharmingen 01541D, new = 553250
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD54 / ICAM-1

Antibody against:anti-mouse CD54 / ICAM-1
Label: FITC
Clone: clone 3E2
Isotype: Hamster IgG1, k
Type: commercial
Source: Pharmingen 553252
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD54 / ICAM-1

Antibody against:anti-mouse CD54 / ICAM-1
Label: PE
Clone: clone 3E2
Isotype: Hamster IgG1, k
Type: commercial
Source: BD Biosciences 553253
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD61

Antibody against:anti-mouse CD61
Label: FITC
Clone: 2C9.G2
Isotype: Hamster IgG1, k
Type: commercial
Source: Pharmingen 22444D, new = 553346
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD61 / integrin Beta3

Antibody against:anti-mouse CD61 / integrin Beta3
Label: Biotin
Clone: 2C9.G2
Isotype: Hamster IgG1, k
Type: commercial
Source: Pharmingen 01862D, new = 553345
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD62L

Antibody against:anti-mouse CD62L
Label: PE
Clone: MEL-14
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 104408
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD62P / P-selectin

Antibody against:anti-mouse CD62P / P-selectin
Label: Biotin
Clone: RB40.34
Isotype: Rat IgG1, lambda
Type: commercial
Source: Pharmingen 553743
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD71/transferrin R

Antibody against:anti-mouse CD71/transferrin R
Label: none
Clone: C2
Isotype: Rat IgG1, k
Type: commercial
Source: Pharmingen 01591D, new = 553264
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD80

Antibody against:anti-mouse CD80
Label: Biotin
Clone: 16-10A1
Isotype: Hamster IgG
Type: commercial
Source: BioLegend 104703
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD80

Antibody against:anti-mouse CD80
Label: PE
Clone: 16-10A1
Isotype: Hamster IgG
Type: commercial
Source: BioLegend 104708
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD86

Antibody against:anti-mouse CD86
Label: Biotin
Clone: PO3
Isotype: ? (discontinued)
Type: commercial
Source: BioLegend 105103 (discontinued)
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD86

Antibody against:anti-mouse CD86
Label: PE
Clone: PO3
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 105106
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD86

Antibody against:anti-mouse CD86
Label: FITC
Clone: GL1
Isotype: Rat IgG2a, k
Type: commercial
Source: Pharmingen 09274D, new = 553691
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD90.1 / anti-rat CD90 (Thy-1.1)

Antibody against:anti-mouse CD90.1 / anti-rat CD90 (Thy-1.1)
Label: PE
Clone: OX-7
Isotype: Mouse IgG1, k
Type: commercial
Source: BD 551401
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD90.2 / Thy-1.2

Antibody against:anti-mouse CD90.2 / Thy-1.2
Label: FITC
Clone: 53-2.1
Isotype: Rat IgG2a, k
Type: commercial
Source: Pharmingen 553003
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD95 / Fas

Antibody against:anti-mouse CD95 / Fas
Label: FITC
Clone: Jo2
Isotype: Hamster IgG2
Type: commercial
Source: BD Pharmingen 554257
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD103

Antibody against:anti-mouse CD103
Label: PerCP-Cy5.5
Clone: clone 2E7
Isotype: Hamster IgG
Type: commercial
Source: BioLegend 121416
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD106 / VCAM-1

Antibody against:anti-mouse CD106 / VCAM-1
Label: FITC
Clone: 429/MVCAM.A
Isotype: Rat IgG2a, k
Type: commercial
Source: Pharmingen 01814D, new = 553332
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD107a / LAMP-1

Antibody against:anti-mouse CD107a / LAMP-1
Label: FITC
Clone: 1D4B
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 121605
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD117

Antibody against:anti-mouse CD117
Label: PE
Clone: 2B8
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 105807
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD119 IFNgR alpha

Antibody against:anti-mouse CD119 IFNgR alpha
Label: Biotin
Clone: GR20
Isotype: Rat IgG2a, k
Type: commercial
Source: Pharmingen 558771
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD122 / IL-2R Beta

Antibody against:anti-mouse CD122 / IL-2R Beta
Label: PE
Clone: TM-Beta1
Isotype: Rat IgG2b, k
Type: commercial
Source: BD Pharmingen 553362
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD122 / IL-2R Beta

Antibody against:anti-mouse CD122 / IL-2R Beta
Label: FITC
Clone: TM-Beta1
Isotype: Rat IgG2b, k
Type: commercial
Source: Pharmingen 553361
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD127 / IL-7Ra

Antibody against:anti-mouse CD127 / IL-7Ra
Label: FITC
Clone: A7R34
Isotype: Rat IgG2a, k
Type: commercial
Source: eBiosciences 11-1271-82
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD137 / 4-1BB

Antibody against:anti-mouse CD137 / 4-1BB
Label: PE
Clone: 17B5
Isotype: Hamster IgG
Type: commercial
Source: eBioscience 12-1371-83
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD152

Antibody against:anti-mouse CD152
Label: PE
Clone: UC10-4B9
Isotype: HamsterIgG
Type: commercial
Source: BioLegend 106306
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD172a / SIRPa

Antibody against:anti-mouse CD172a / SIRPa
Label: APC
Clone: P84
Isotype: Rat IgG1, k
Type: commercial
Source: BD Pharmingen 560106
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD178 / FasL

Antibody against:anti-mouse CD178 / FasL
Label: PE
Clone: MFL3
Isotype: Hamster IgG
Type: commercial
Source: BioLegend 106606
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD205 / DEC205

Antibody against:anti-mouse CD205 / DEC205
Label: FITC
Clone: NLDC-145
Isotype: Rat IgG2a
Type: commercial
Source: Cedarlane CL89145F
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD210/ IL-10R

Antibody against:anti-mouse CD210/ IL-10R
Label: PE
Clone: 1B1.3A
Isotype: Rat IgG1, k
Type: commercial
Source: Pharmingen 559914
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD274 / PD-L1 / B7-H1

Antibody against:anti-mouse CD274 / PD-L1 / B7-H1
Label: PE
Clone: MIH5
Isotype: Rat IgG2a, l
Type: commercial
Source: eBioscience 12-5982-83
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD282 / TLR2

Antibody against:anti-mouse CD282 / TLR2
Label: FITC
Clone: T2.5
Isotype: Mouse IgG1, k
Type: commercial
Source: BioLegend 121805
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD284 / TLR4/MD2

Antibody against:anti-mouse CD284 / TLR4/MD2
Label: PE
Clone: MTS510
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 117605
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD314 / NKG2D

Antibody against:anti-mouse CD314 / NKG2D
Label: PE
Clone: C7
Isotype: Hamster IgG
Type: commercial
Source: BioLegend 115705
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD335 / NKp46

Antibody against:anti-mouse CD335 / NKp46
Label: PE
Clone: 29A1.4
Isotype: Rat IgG2a, k
Type: commercial
Source: eBioscience 12-3351-82
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CD335 / NKp46

Antibody against:anti-mouse CD335 / NKp46
Label: APC
Clone: 29A1.4
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 137607
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CLTA-4 / CD152

Antibody against:anti-mouse CLTA-4 / CD152
Label: none (LEAF-purified)
Clone: 9H10
Isotype: Hamster IgG
Type: commercial
Source: BioLegend 106203
Used for: neutralization / blocking in vivo
Treatment / Staining Regime: 200 ug i.p. on day +3, +6, +9 and every 3 days thereafter.
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse complement C3

Antibody against:anti-mouse complement C3
Label: FITC
Clone: polyclonal
Isotype: Goat IgG
Type: commercial
Source: ICN / Cappel 55500
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CTLA-4 / CD152

Antibody against:anti-mouse CTLA-4 / CD152
Label: none
Clone: 9D9
Isotype: Syrian hamster
Type: commercial
Source: Bristol Myers Squibb
Used for: neutralization / blocking in vivo
Treatment / Staining Regime: 200 ug i.p. on day +3, +6, +9 and every 3 days thereafter.
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse CXCR3

Antibody against:anti-mouse CXCR3
Label: none
Clone: CXCR3-173
Isotype: A. Hamster IgG
Type: purified hybridoma supernatant
Source:
Used for: neutralization / blocking in vivo
Treatment / Staining Regime: 200-500 ug ip qed
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse DR5 / CD262

Antibody against:anti-mouse DR5 / CD262
Label: Biotin
Clone: MD5-1
Isotype: Hamster IgG
Type: commercial
Source: eBiosciences 13-5883-82
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse F4/80

Antibody against:anti-mouse F4/80
Label: PE
Clone: BM8
Isotype: Rat IgG2a
Type: commercial
Source: Caltag MF48004
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse F4/80

Antibody against:anti-mouse F4/80
Label: FITC
Clone: CI:A3-1
Isotype: Rat IgG2b
Type: commercial
Source: Caltag RM2901
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse F4/80

Antibody against:anti-mouse F4/80
Label: PerCP
Clone: BM8
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegend 123125
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse FAP

Antibody against:anti-mouse FAP
Label: none
Clone: polyclonal
Isotype: Rabbit polyclonal
Type: commercial
Source: abcam ab53066
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse FOXP3

Antibody against:anti-mouse FOXP3
Label: APC
Clone: FKJ-16s
Isotype: Rat IgG2a, k
Type: commercial
Source: eBiosciences 17-5773-82
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse FOXP3

Antibody against:anti-mouse FOXP3
Label: PE
Clone: MF-14
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 126403
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse H-2Db

Antibody against:anti-mouse H-2Db
Label: Biotin
Clone: 34-2-12
Isotype: Mouse IgG2a, k
Type: commercial
Source: Pharmingen 06132D, new = 553578
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse H-2Db

Antibody against:anti-mouse H-2Db
Label: none (no azide / low endotoxin)
Clone: 28-14-8
Isotype: Mouse IgG2a, k
Type: commercial
Source: Pharmingen 0623OD, new = 559579
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse H-2Db

Antibody against:anti-mouse H-2Db
Label: PE
Clone: KH95
Isotype: Mouse IgG2a, k
Type: commercial
Source: BD Pharmingen 553574
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse H-2Db / H-2Ld

Antibody against:anti-mouse H-2Db / H-2Ld
Label: none
Clone: 28-14-8
Isotype: Mouse IgG2a, k
Type: commercial
Source: BioLegend 114502
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse H-2Kb

Antibody against:anti-mouse H-2Kb
Label: none
Clone: AF6-88.5
Isotype: Mouse IgG2a, k
Type: commercial
Source: Pharmingen 553567
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse H-2Kb

Antibody against:anti-mouse H-2Kb
Label: none
Clone: AF6-88.5
Isotype: Mouse IgG2a, k
Type: commercial
Source: BioLegend 116502
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse H-2Kb

Antibody against:anti-mouse H-2Kb
Label: Biotin
Clone: AF6-88.5
Isotype: Mouse IgG2a, k
Type: commercial
Source: Pharmingen 06102D, new = 553568
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse H-2Kb

Antibody against:anti-mouse H-2Kb
Label: PE
Clone: AF6-88.5
Isotype: Mouse IgG2a, k
Type: commercial
Source: BD Pharmingen 553570
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse H-2Kd

Antibody against:anti-mouse H-2Kd
Label: PE
Clone: SF1-1.1
Isotype: Mouse IgG2a, k
Type: commercial
Source: BD Pharmingen 553566
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse H-2Kd

Antibody against:anti-mouse H-2Kd
Label: Biotin
Clone: SF1-1.1
Isotype: Mouse IgG2a, k
Type: commercial
Source: Pharmingen 06092D, new = 553564
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse H-2Kk

Antibody against:anti-mouse H-2Kk
Label: PE
Clone: 36-7-5
Isotype: Mouse IgG2a, k
Type: commercial
Source: BD Pharmingen 553593
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse H-2Kk

Antibody against:anti-mouse H-2Kk
Label: Biotin
Clone: 36-7-5
Isotype: Mouse IgG2a, k
Type: commercial
Source: Pharmingen 06182D, new = 553591
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse H60

Antibody against:anti-mouse H60
Label: none
Clone: polyclonal
Isotype: Goat polyclonal
Type: commercial
Source: Santa Cruz sc-20330
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse I-Ab

Antibody against:anti-mouse I-Ab
Label: Biotin
Clone: AF6-120.1
Isotype: IgG2a, k
Type: commercial
Source: Pharmingen 553550
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse I-Ab

Antibody against:anti-mouse I-Ab
Label: PE
Clone: AF6-120.1
Isotype: Mouse IgG2a, k
Type: commercial
Source: BioLegend 116408
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse I-Ab

Antibody against:anti-mouse I-Ab
Label: FITC
Clone: AF6-120.1
Isotype: Mouse IgG2a, k
Type: commercial
Source: BD Pharmingen 553551
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse I-Ad

Antibody against:anti-mouse I-Ad
Label: PE
Clone: AMS-32.1
Isotype: Mouse IgG2b, k
Type: commercial
Source: BD Pharmingen 553548
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse I-Ek

Antibody against:anti-mouse I-Ek
Label: Biotin
Clone: 14-4-45
Isotype: Mouse IgG2a, k
Type: commercial
Source: Pharmingen 06022A, new = 558735
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IFNAR1

Antibody against:anti-mouse IFNAR1
Label: none
Clone: MAR1-5A3
Isotype: Mouse IgG1, k
Type: purified hybridoma supernatant
Source:
Used for: neutralization / blocking in vivo
Treatment / Staining Regime: 2.5 mg i.p. on day -1, and 0.5 mg weekly maintenance dose.
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IFNg

Antibody against:anti-mouse IFNg
Label: PE
Clone: XMG1.2
Isotype: Rat IgG1, k
Type: commercial
Source: BD Biosciences554412
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IFNg

Antibody against:anti-mouse IFNg
Label: none
Clone: H22
Isotype: A.Hamster IgG
Type: purified hybridoma supernatant
Source:
Used for: neutralization in vivo
Treatment / Staining Regime: 750 ug i.p. on day -1, and 250 ug weekly maintenance dose.
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse Ig

Antibody against:anti-mouse Ig
Type: Secondary Antibody
Label: PE
Clone: polyclonal
Isotype: Goat
Type: commerciai
Source: Pharmingen 550589
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse Ig

Antibody against:anti-mouse Ig
Type: Secondary Antibody
Label: FITC
Clone: polyclonal
Isotype: Goat
Type: commercial
Source: Pharmingen 12064D, new = 554001
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IgD

Antibody against:anti-mouse IgD
Type: Secondary Antibody
Label: FITC
Clone: 11-26c.2a
Isotype: Rat IgG2a, k
Type: commercial
Source: Pharmingen 553439
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IgDb

Antibody against:anti-mouse IgDb
Label: FITC
Clone: 217-170
Isotype: Mouse IgG1a, k
Type: commercial
Source: Pharmingen 05074D
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IgG

Antibody against:anti-mouse IgG
Type: Secondary Antibody
Label: PE
Clone: polyclonal
Isotype: Goat
Type: commerciai
Source: Caltag M300004-1
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IgG

Antibody against:anti-mouse IgG
Type: Secondary Antibody
Label: AlexaFluor 488
Clone: polyclonal
Isotype: Goat
Type: commerciai
Source: Molecular Probes A11029
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IgG2a

Antibody against:anti-mouse IgG2a
Type: Secondary Antibody
Label: FITC
Clone: ?
Isotype: ?
Type: commercial
Source: Pharmingen 02014D
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IgM

Antibody against:anti-mouse IgM
Type: Secondary Antibody
Label: FITC
Clone: ?
Isotype: ?
Type: commercial
Source: Pharmingen 02204D
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IgM

Antibody against:anti-mouse IgM
Type: Secondary Antibody
Label: PE
Clone: R6-60.2
Isotype: Rat IgG2a, k
Type: commercial
Source: Pharmingen 553409, old = 02084D
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IL-1a

Antibody against:anti-mouse IL-1a
Label: none
Clone: ALF-161
Isotype: A.Hamster IgG
Type: purified hybridoma supernatant
Source:
Used for: neutralization in vivo
Treatment / Staining Regime: 200 ug
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IL-1a

Antibody against:anti-mouse IL-1a
Label: PE
Clone: ALF-161
Isotype: Hamster IgG
Type: commercial
Source: BioLegend 503203
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IL-1B

Antibody against:anti-mouse IL-1B
Label: none
Clone: B122
Isotype: A.Hamster IgG
Type: purified hybridoma supernatant
Source:
Used for: neutralization in vivo
Treatment / Staining Regime: 200 ug
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IL-1R1

Reference:

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anti-mouse IL-2

Antibody against:anti-mouse IL-1R1
Label: none
Clone: JAMA-147
Isotype: Hamster IgG
Type: purified hybridoma supernatant
Source:
Used for: neutralization in vivo
Treatment / Staining Regime: 200 ug
Performance:
Information provided by the lab of: Robert Schreiber
Antibody against:anti-mouse IL-2
Label: PE
Clone: JES6-5H4
Isotype: Rat IgG2b
Type: commercial
Source: Pharmingen 18175A
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IL-4

Antibody against:anti-mouse IL-4
Label: APC
Clone: 11B11
Isotype: Rat IgG1, k
Type: commercial
Source: BioLegend 504105
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IL-5

Antibody against:anti-mouse IL-5
Label: APC
Clone: TRFK5
Isotype: Rat IgG1, k
Type: commercial
Source: BioLegend 504305
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IL-6

Antibody against:anti-mouse IL-6
Label: PE
Clone: MP5-20F3
Isotype: Rat IgG1, k
Type: commercial
Source: BioLegend 504503
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IL-10

Antibody against:anti-mouse IL-10
Label: PE
Clone: JES5-16E3
Isotype: Rat IgG2b
Type: commercial
Source: BD Biosciences 554467
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IL-10R

Antibody against:anti-mouse IL-10R
Label: Biotin
Clone: 1B1.3a
Isotype: Rat IgG1, k
Type: commercial
Source: Pharmingen 559913
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IL-12

Antibody against:anti-mouse IL-12
Label: PE
Clone: C15.6
Isotype: Rat IgG1, k
Type: commercial
Source: BioLegend 505203
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IL-12p40

Antibody against:anti-mouse IL-12p40
Label: none
Clone: C17.8
Isotype: Rat IgG2a
Type: purified hybridoma supernatant
Source:
Used for: neutralization in vivo
Treatment / Staining Regime: 750 ug i.p. on day -1, and 250 ug bi-weekly maintenance dose.
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse IL-17

Antibody against:anti-mouse IL-17
Label: PE
Clone: TC11-18H10.1
Isotype: Rat IgG1, k
Type: commercial
Source: BioLegend 506904
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse Ly-6A/E / SCA-1

Antibody against:anti-mouse Ly-6A/E / SCA-1
Label: PE
Clone: E13-161.7
Isotype: Rat IgG2a, k
Type: commercial
Source: Pharmingen 01835B, new = 553336
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse Ly-6G / Ly-6C (Gr-1)

Antibody against:anti-mouse Ly-6G / Ly-6C (Gr-1)
Label: none
Clone: RB6-8C5
Isotype: Rat IgG2b, k
Type: commercial
Source: Pharmingen 01211D, new = 553123
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse Ly-51

Antibody against:anti-mouse Ly-51
Label: PE
Clone: 6C3/BP-1
Isotype: Mouse IgG2a, k
Type: commercial
Source: BD Pharmingen 553735
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse Ly6G

Antibody against:anti-mouse Ly6G
Label: PE
Clone: RB6-8C5
Isotype: Rat IgG2b, k
Type: commercial
Source: Pharmingen 553128
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse Ly6G

Antibody against:anti-mouse Ly6G
Label: PE
Clone: 1A8
Isotype: Rat IgG2a, k
Type: commercial
Source: BD Pharmingen 551461
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse Ly6G/Ly6C (Gr-1)

Antibody against:anti-mouse Ly6G/Ly6C (Gr-1)
Label: PerCP-Cy5.5
Clone: RB6-8C5
Isotype: Rat IgG2b, k
Type: commercial
Source: Pharmingen 552093
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse Ly6G/Ly6C (Gr-1)

Antibody against:anti-mouse Ly6G/Ly6C (Gr-1)
Label: none
Clone: RB6-8C5
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 108414
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse Ly6G/Ly6C (Gr-1)

Antibody against:anti-mouse Ly6G/Ly6C (Gr-1)
Label: APC
Clone: RB6-8C5
Isotype:
Type: commercial
Source: Pharmingen 553129
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse Ly6G/Ly6C (Gr-1)

Antibody against:anti-mouse Ly6G/Ly6C (Gr-1)
Label: FITC
Clone: RB6-8C5
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 108406
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse MAdCAM-1

Antibody against:anti-mouse MAdCAM-1
Label: none
Clone: MECA-367
Isotype: Rat IgG2a, k
Type: commercial
Source: Pharmingen 09721D
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse NK1.1

Antibody against:anti-mouse NK1.1
Label: PE
Clone: PK136
Isotype: Mouse IgG2a, k
Type: commercial
Source: BD Pharmingen 553165
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse NK1.1

Antibody against:anti-mouse NK1.1
Label: APC
Clone: PK136
Isotype: Mouse IgG2a, k
Type: commercial
Source: BioLegend 108710
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse NK1.1

Antibody against:anti-mouse NK1.1
Label: none
Clone: PK136
Isotype: Mouse IgG2a, k
Type: purified hybridoma supernatant
Source:
Used for: depletion in vivo
Treatment / Staining Regime: 200 ug i.p. on days -2, 0, +2, and 100 ug at 5-day intervals thereafter.
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse NKG2D / CD314

Antibody against:anti-mouse NKG2D / CD314
Label: none
Clone: C7
Isotype: Hamster IgG
Type: purified hybridoma supernatant
Source:
Used for: neutralization / blocking in vivo
Treatment / Staining Regime: 750 ug i.p. on day -1, and 250 ug bi-weekly maintenance dose.
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse pan-NK cells

Antibody against:anti-mouse pan-NK cells
Label: Biotin
Clone: DX5
Isotype: Rat IgM, k
Type: commercial
Source: Pharmingen 553856
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse PD-1 / CD279

Antibody against:anti-mouse PD-1 / CD279
Label: none
Clone: 4H2
Isotype: Rat-mouse chimera
Type: commercial
Source: Bristol Myers Squibb
Used for: neutralization / blocking in vivo
Treatment / Staining Regime: 200 ug i.p. on day +3, +6, +9 and every 3 days thereafter.
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse PD-1 / CD279

Antibody against:anti-mouse PD-1 / CD279
Label: none (LEAF-purified)
Clone: RMP1-14
Isotype: Rat IgG2a, k
Type: commercial
Source: BioLegned 114107
Used for: neutralization / blocking in vivo
Treatment / Staining Regime: 200 ug i.p. on day +3, +6, +9 and every 3 days thereafter.
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse PD-L1 / CD274

Antibody against:anti-mouse PD-L1 / CD274
Label: none
Clone: 14D8ch
Isotype: Rat-mouse chimera
Type: commercial
Source: Bristol Myers Squibb
Used for: neutralization / blocking in vivo
Treatment / Staining Regime: 200 ug i.p. on day +3, +6, +9 and every 3 days thereafter.
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse PD-L1 / CD274

Antibody against:anti-mouse PD-L1 / CD274
Label: none (LEAF-purified)
Clone: 10F.9G2
Isotype: Rat IgG2b, k
Type: commercial
Source: BioLegend 124303
Used for: neutralization / blocking in vivo
Treatment / Staining Regime: 200 ug i.p. on day +3, +6, +9 and every 3 days thereafter.
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TCR alpha/Beta

Antibody against:anti-mouse TCR alpha/Beta
Label: Biotin
Clone: H57-597
Isotype: Hamster IgG2, lambda
Type: commercial
Source: Pharmingen 01302A, new = 553168
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TCR gamma delta

Antibody against:anti-mouse TCR gamma delta
Label: Biotin
Clone: GL3
Isotype: Hamster IgG2, k
Type: commercial
Source: Pharmingen 01312D, new = 553176
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TCR Vbeta 5.1/2

Antibody against:anti-mouse TCR Vbeta 5.1/2
Label: PE
Clone: MR9-4
Isotype: Mouse IgG1, k
Type: commercial
Source: BD Pharmingen 553190
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TCR Vbeta 8.1/2

Antibody against:anti-mouse TCR Vbeta 8.1/2
Label: PE
Clone: MR5-2
Isotype: Mouse IgG2a, k
Type: commercial
Source: BD Pharmingen 553186
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TCR Vg3

Antibody against:anti-mouse TCR Vg3
Label: PE
Clone: 536
Isotype: Hamster IgG1, k
Type: commercial
Source: BD Pharmingen 01485A, new = 553230
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TCRBeta

Antibody against:anti-mouse TCRBeta
Label: APC
Clone: H57-597
Isotype: Hamster IgG
Type: commercial
Source: BioLegend 109212
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TCRgd

Antibody against:anti-mouse TCRgd
Label: PE
Clone: GL3
Isotype: Hamster IgG
Type: commercial
Source: BioLegend 118107
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TCRgd

Antibody against:anti-mouse TCRgd
Label: PE
Clone: V65
Isotype: Mouse IgG1, k
Type: commercial
Source: BD Pharmingen 551802
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TCRVbeta 8.3

Antibody against:anti-mouse TCRVbeta 8.3
Label: PE
Clone: 1B3.3
Isotype: Hamster IgG3
Type: commercial
Source: BD Pharmingen 553664
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TLR3

Antibody against:anti-mouse TLR3
Label: none
Clone: polyclonal
Isotype: Rat serum
Type: commercial
Source: eBiosciences 24-9031-91
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TLR5

Antibody against:anti-mouse TLR5
Label: PE
Clone: 85B 152.5
Isotype: Mouse IgG2a, k
Type: commercial
Source: Imgenex IMG-663D
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TLR9

Antibody against:anti-mouse TLR9
Label: FITC
Clone: 26C593.2
Isotype: Mouse IgG, k
Type: commercial
Source: Imgenex IMG-305C
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TNFa

Antibody against:anti-mouse TNFa
Label: PE
Clone: MP6-XT22
Isotype: Rat IgG1, k
Type: commercial
Source: Pharmingen 554419
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TNFa

Antibody against:anti-mouse TNFa
Label: none
Clone: TN3-19.12
Isotype: A. Hamster IgG
Type: purified hybridoma supernatant
Source:
Used for: neutralization in vivo
Treatment / Staining Regime: 300 ug i.p. weekly. (200/250 ug)
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TNFR1

Antibody against:anti-mouse TNFR1
Label:
Clone: 55R-593
Isotype: A. Hamster IgG
Type: purified hybridoma supernatant
Source:
Used for: agonist Ab
Treatment / Staining Regime: 250 ug
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TNFR1

Antibody against:anti-mouse TNFR1
Label:
Clone: 55R-170
Isotype: A. Hamster IgG
Type: purified hybridoma supernatant
Source:
Used for: antagonist Ab
Treatment / Staining Regime: 250 ug
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TNFR2

Antibody against:anti-mouse TNFR2
Label:
Clone: TR75-54
Isotype: A. Hamster IgG
Type: purified hybridoma supernatant
Source:
Used for: antagonist, ELISA capture
Treatment / Staining Regime: 250 ug
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TNFR2

Antibody against:anti-mouse TNFR2
Label:
Clone: TR75-32
Isotype: A. Hamster IgG
Type: purified hybridoma supernatant
Source:
Used for: antagonist, ELISA detection
Treatment / Staining Regime: 250 ug
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-mouse TRAIL / CD253

Antibody against:anti-mouse TRAIL / CD253
Label: none
Clone: N2B2
Isotype: Rat IgG2a
Type: purified hybridoma supernatant
Source:
Used for: neutralization / blocking in vivo
Treatment / Staining Regime: 750 ug i.p. on day -1, and 250 ug bi-weekly maintenance dose.
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-rabbit IgG

Antibody against:anti-rabbit IgG
Type: Secondary Antibody
Label: FITC
Clone: polyclonal
Isotype: Goat
Type: commercial
Source: eBioscience 11-4839-81
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-rat Ig

Antibody against:anti-rat Ig
Type: Secondary Antibody
Label: FITC
Clone: polyclonal
Isotype: ?
Type: commercial
Source: Pharmingen 12114D
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-rat IgG

Antibody against:anti-rat IgG
Type: Secondary Antibody
Label: PE
Clone: polyclonal
Isotype: Goat
Type: commerciai
Source: Southern Biologicals 3030-09
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-rat IgG

Antibody against:anti-rat IgG
Label: none
Clone: poly 4054
Isotype: Goat
Type: commercial
Source: BioLegend 405401
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-rat IgG

Antibody against:anti-rat IgG
Type: Secondary Antibody
Label: FITC
Clone: polyclonal
Isotype: Goat
Type: commercial
Source: BioLegend 405404
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-rat IgG (H+L)

Antibody against:anti-rat IgG (H+L)
Label: Biotin
Clone:
Isotype: Rabbit
Type: commercial
Source: Vector Laboratories BA-4001
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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anti-rat IgG2a

Antibody against:anti-rat IgG2a
Type: Secondary Antibody
Label: FITC
Clone: MRG2a-83
Isotype: Mouse IgG
Type: commercial
Source: BioLegend 407505
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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CD1d

Antibody against:CD1d
Label: PE-Cy7
Clone: GK1.5
Isotype: rat IgG2b
Type: commercial/purified
Source: eBioscience cat# 25-0041-81
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD3

Antibody against:CD3
Label: APC
Clone: A20
Isotype: mouse IgG2a k
Type: commercial/purified
Source: eBioscience cat# 17-0453-81
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: 3 log shift
Information provided by the lab of: Ian Frazer

Reference:

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CD3e

Antibody against:CD3e
Label: PE
Clone: 104
Isotype: mouse IgG2a k
Type: commercial/purified
Source: eBioscience cat# 12-0454-81
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: 2 log shift
Information provided by the lab of: Ian Frazer

Reference:

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CD3e

Antibody against:CD3e
Label: APC
Clone: 104
Isotype: mouse IgG2a k
Type: commercial/purified
Source: eBioscience cat# 17-0454-82
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD3e

Antibody against:CD3e
Label: PE
Clone: MEL-14
Isotype: rat IgG2a
Type: commercial/purified
Source: eBioscience cat# 12-0621-82
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: 2 log shift
Information provided by the lab of: Ian Frazer

Reference:

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CD4

Antibody against:CD4
Label: FITC
Clone: 53-6.7
Isotype: rat IgG2a
Type: commercial/purified
Source: eBioscience cat# 11-0081
Restrictions on Use:
Used for:
Treatment / Staining Regime: 0.5ug
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD4

Antibody against:CD4
Label: PE
Clone: 53-6.7
Isotype: rat IgG2a
Type: commercial/purified
Source: eBioscience cat# 12-0081-83
Restrictions on Use:
Used for: IF, FACS
Treatment / Staining Regime: 0.2ug
Performance: 3 log shift
Information provided by the lab of: Ian Frazer

Reference:

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CD4

Antibody against:CD4
Label: nil
Clone: 53-5.8
Isotype: IgG1
Type: purified hybridoma supernatant
Source: in-house from hybridoma
Restrictions on Use: no
Used for: in-vivo depletion
Treatment / Staining Regime: Treat mice IP Day 0 100ug, Day 2 150ug, Day 9 100ug, test depletion Day 12. [Azad], Or Treat IP Day 0 150ug, Day 2 100ug, Day 7 100ug, test depletion Day10, plus weekly 100ug shots at Day
Performance: 90% depletion of CD8 splenocytes in vivo. Maintain with weekly 100ug shots for long-term expts
Information provided by the lab of: Ian Frazer

Reference:

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CD4

Antibody against:CD4
Label: PE-Cy5.5
Clone: 53-6.7
Isotype: rat IgG2a, k
Type: commercial/purified
Source: Biolegend cat# 100720
Restrictions on Use:
Used for: IF
Treatment / Staining Regime: 0.2ug
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD4

Antibody against:CD4
Label: APC
Clone: 53-6.7
Isotype: rat IgG2a, ak
Type: commercial/purified
Source: eBioscience cat# 17-0081-83
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: 2.5 log shift
Information provided by the lab of: Ian Frazer

Reference:

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CD4 (L3T4)

Antibody against:CD4 (L3T4)
Label: FITC
Clone: MOMA-2
Isotype: Rat IgG2b
Type: commercial/purified
Source: Serotec #MCA519F
Restrictions on Use:
Used for: IF
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD4 (L3T4)

Antibody against:CD4 (L3T4)
Label: Biotin
Clone: BM8
Isotype: rat IgG2a
Type: commercial/purified
Source: eBioscience cat# 13-4801-82
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.5ug
Performance: 0.5- 1 log
Information provided by the lab of: Ian Frazer

Reference:

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CD4 (L3T4)

Antibody against:CD4 (L3T4)
Label: FITC
Clone: BM8
Isotype: rat IgG2a, k
Type: commercial/purified
Source: eBioscience cat# 11-4801-82
Restrictions on Use:
Used for: IF
Treatment / Staining Regime: 0.5ug
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD4 (L3T4)

Antibody against:CD4 (L3T4)
Label: nil
Clone: 9H10
Isotype:
Type:
Source:
Restrictions on Use: MTA James Allison; Howard Hughes Inst, CA
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD8

Antibody against:CD8
Label: nil
Clone: 10F.9G2
Isotype:
Type: commercial purified
Source: BioXcell
Restrictions on Use: Permission to purchase Ab from Hideo Yagito required
Used for: in vivo blocking
Treatment / Staining Regime: 200ug IP
Performance: 0.6
Information provided by the lab of: Ian Frazer

Reference:

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CD8a

Antibody against:CD8a
Label: nil
Clone: TY25
Isotype:
Type:
Source: Hideo Yagita
Restrictions on Use: Permission to use Ab from Hideo Yagito required
Used for: in vivo blocking
Treatment / Staining Regime: 200ug IP
Performance: 1
Information provided by the lab of: Ian Frazer

Reference:

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CD8a (Ly-2)

Antibody against:CD8a (Ly-2)
Label: FITC
Clone: MR11-1
Isotype: mouse IgG1
Type: commercial/purified
Source: BD Pharmingen cat# 553300
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.5ug
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD8a (Ly-2)

Antibody against:CD8a (Ly-2)
Label: PE-Cy7
Clone: M1/70
Isotype: Rat IgG2b
Type: commercial
Source: eBioScience # 25-0112
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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CD8a (Ly-2)

Antibody against:CD8a (Ly-2)
Label: FITC
Clone: H57-597
Isotype: hamster IgG2
Type: commercial/purified
Source: BD Pharmingen cat# 553171
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.5ug
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD8a (Ly-2)

Antibody against:CD8a (Ly-2)
Label: purified
Clone: MR5D3
Isotype: Rat IgG2A
Type: Biolegend
Source: 123009
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 5ug/ml
Performance: good
Information provided by the lab of: Matthew Krummel

Reference:

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CD8a (Ly-2)

Antibody against:CD8a (Ly-2)
Label: PE
Clone: M290
Isotype: rat IgG2a
Type: commercial/purified
Source: BD Pharmingen cat# 557495
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.5ug
Performance: 0.5- 1 log shift
Information provided by the lab of: Ian Frazer

Reference:

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CD8a (Ly-2)

Antibody against:CD8a (Ly-2)
Label: purified
Clone: 927
Isotype: Rat IgG2b
Type: eBioscience
Source: 51-3172-80
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 2ug/ml
Performance: good
Information provided by the lab of: Matthew Krummel

Reference:

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CD8a (Ly-2)

Antibody against:CD8a (Ly-2)
Label: purified
Clone: AL-21
Isotype: Rat
Type: BD Pharmingen
Source: 553104
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 5ug/ml
Performance: good
Information provided by the lab of: Matthew Krummel

Reference:

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CD8b

Antibody against:CD8b
Label: FITC
Clone: HL3
Isotype: hamster IgG
Type: commercial
Source: BD Pharmingen cat# 557400
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2 ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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CD11b

Antibody against:CD11b
Label: nil
Clone: 20H2
Isotype:
Type: purified hybridoma supernatant
Source: M. Kronenberg, La Jolla, CA
Restrictions on Use: used with permission
Used for: in-vivo CD1d blockade
Treatment / Staining Regime: in-vivo blockade for skin grafting expt- Donor ear skin treated D -1 ID 200ug Ab 100ul vol. Host blockade 200ug SC at graft site twice weekly for 4 weeks.
Performance: 50-65% reduction in CD1d mean fluorescence intensity by FACS- blood circulating lymphocytes
Information provided by the lab of: Ian Frazer

Reference:

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CD11b

Antibody against:CD11b
Label: nil
Clone: PC61
Isotype:
Type:
Source:
Restrictions on Use:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD11c

Antibody against:CD11c
Label: Purified
Clone: 17A2
Isotype: rat IgG2b,k
Type: commercial/purified
Source: eBioscience cat# 14-0032-85
Restrictions on Use:
Used for: IF
Treatment / Staining Regime: 0.5ug
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD11c

Antibody against:CD11c
Label: APC
Clone: PC61
Isotype: rat IgG1
Type: commercial/purified
Source: BD Pharmingen cat# 557192
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD11c

Antibody against:CD11c
Label: Biotin
Clone: TY25
Isotype: rat IgG2a
Type: commercial/purified
Source: Biolegend cat# 107203
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.5ug
Performance: 2 log shift
Information provided by the lab of: Ian Frazer

Reference:

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CD16/CD32 (Fc block)

Antibody against:CD16/CD32 (Fc block)
Label: PE
Clone: G8.8
Isotype: rat IgG2a
Type: commercial/purified
Source: Biolegend cat# 118205
Restrictions on Use:
Used for: IF, FACS
Treatment / Staining Regime: 0.2ug
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD19

Antibody against:CD19
Label: PerCP
Clone: 145-2C11
Isotype: hamster IgG1
Type: commercial/purified
Source: BD Pharmingen cat# 553067
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: 2 log shift
Information provided by the lab of: Ian Frazer

Reference:

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CD25

Antibody against:CD25
Label: APC
Clone: RM4-5
Isotype: rat IgG2a
Type: commercial/purified
Source: eBioscience cat# 17-0042-81
Restrictions on Use:
Used for: IF, FACS
Treatment / Staining Regime: 0.2ug
Performance: 2 log shift
Information provided by the lab of: Ian Frazer

Reference:

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CD25

Antibody against:CD25
Label: PE
Clone: GK1.5
Isotype: rat IgG2b
Type: commercial/purified
Source: eBioscience cat# 12-0041-85
Restrictions on Use:
Used for: IF, FACS
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD25

Antibody against:CD25
Label: nil
Clone: FGK
Isotype: rat IgG2a
Type:
Source: Prof Antionius Rolink (Basel, Switzerland)
Restrictions on Use: used with permission
Used for: in-vivo activating (agonist?)
Treatment / Staining Regime: in-vivo 150ug IV(?) on Day 0 then every 5 days
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD25 (IL-2R)

Antibody against:CD25 (IL-2R)
Label: FITC
Clone: IM7
Isotype: rat IgG2b k
Type: commercial/purified
Source: BD Pharmingen cat# 553133
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.5ug
Performance: 2 log shift
Information provided by the lab of: Ian Frazer

Reference:

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CD40

Antibody against:CD40
Label: PE
Clone: FJK-16s
Isotype: rat IgG2a, k
Type: commercial/purified
Source: eBioscience cat# 12-5773-82
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug. must use eBio fix/perm buffers.
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD44

Antibody against:CD44
Label: nil
Clone: R4-6A2
Isotype:
Type: purified hybridoma supernatant
Source: in-house from hybridoma
Restrictions on Use:
Used for: in-vivo blocking
Treatment / Staining Regime: in-vivo blocking, 1.0mg SC neck scruff Day 0 and Day 4
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD45 (LCA, Ly-5)

Antibody against:CD45 (LCA, Ly-5)
Label: nil
Clone: GR20
Isotype: R35-95
Type:
Source:
Restrictions on Use:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD45.1

Antibody against:CD45.1
Label: nil
Clone: MAC49
Isotype:
Type: purified hybridoma supernatant
Source: Ray Steptoe, UQDI
Restrictions on Use:
Used for: in-vivo depletion control for CD8b
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD45.1

Antibody against:CD45.1
Label: nil
Clone:
Isotype:
Type: commercial/purified
Source: Bio-Express/ BioXcell
Restrictions on Use:
Used for: in-vivo neutralisation
Treatment / Staining Regime: ?0.5mg?
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD45.2

Antibody against:CD45.2
Label: Alexa Fluor 647
Clone: 929F3.01
Isotype: rat IgG2a
Type: commercial/purified
Source: Dendritics cat# DDX0362A647
Restrictions on Use:
Used for: IF
Treatment / Staining Regime: permeabilise. 0.125- 0.03ug
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD45.2

Antibody against:CD45.2
Label: Biotin
Clone: 205C1
Isotype: rat IgG2a
Type: commercial/purified
Source: Dendritics cat# DDX0370B
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.5ug
Performance: 2 logs over iso
Information provided by the lab of: Ian Frazer

Reference:

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CD45.2

Antibody against:CD45.2
Label: FITC
Clone:
Isotype: rat IgG2b
Type: commercial/purified
Source: eBioscience cat# 11-5321-85
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.125ug for skin, 0.03ug for splenocytes
Performance: 3 logs over iso
Information provided by the lab of: Ian Frazer

Reference:

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CD49b

Antibody against:CD49b
Label: nil
Clone: RMP1-14
Isotype:
Type: commercial purified
Source: BioXcell
Restrictions on Use: Permission to purchase Ab from Hideo Yagito required
Used for: in vivo blocking
Treatment / Staining Regime: 200ug IP
Performance: Not determined
Information provided by the lab of: Ian Frazer

Reference:

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CD62L

Antibody against:CD62L
Label: PE
Clone: MIH5
Isotype: rat IgG2a, lambda
Type: commercial/purified
Source: eBioscience cat# 12-5982-81
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: 1 log over iso
Information provided by the lab of: Ian Frazer

Reference:

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CD103

Antibody against:CD103
Label: PerCP-Cy5.5
Clone: HL3
Isotype: hamster IgG1
Type: commercial/purified
Source: BD Pharmingen cat#560584
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.5ug
Performance: 0.5- 1 log
Information provided by the lab of: Ian Frazer

Reference:

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CD107a (LAMP-1)

Antibody against:CD107a (LAMP-1)
Label: Purified
Clone: 2.4G2
Isotype: rat IgG2b
Type: commercial/purified
Source: BD Pharmingen cat# 553142
Restrictions on Use:
Used for: FACS pre-stain blocking
Treatment / Staining Regime: 0.5ug
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD206

Antibody against:CD206
Label: nil
Clone: GK1.5
Isotype:
Type: purified hybrodoma supernatant
Source: UCSF
Restrictions on Use:
Used for: in-vivo CD4 depletion
Treatment / Staining Regime: in-vivo depletion IP 1mg. One shot only
Performance: Tested 7 days after treatment - 80% depletion
Information provided by the lab of: Ian Frazer

Reference:

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CD273 (PD-L2)

Antibody against:CD273 (PD-L2)
Label: biotin
Clone: A20
Isotype: mouse, IgG2a
Type: commercial/purified
Source: eBioscience cat# 13-0453-85
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.5ug
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CD326 (Ep-CAM)

Antibody against:CD326 (Ep-CAM)
Label: Biotin
Clone: 104
Isotype: mouse IgG2a
Type: commercial/purified
Source: eBioscience cat# 13-0454-82
Restrictions on Use:
Used for: IF, FACS
Treatment / Staining Regime: 0.5ug
Performance:
Information provided by the lab of: Ian Frazer

Reference:

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CTLA4

Antibody against:CTLA4
Label: APC
Clone: PC61.5
Isotype: rat IgG1
Type: commercial
Source: eBioscience cat# 17-0251
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2 ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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F4/80

Antibody against:F4/80
Label: purified
Clone: PC61
Isotype: rat IgG1
Type: UCSF MAB core
Source: UCSF
Restrictions on Use:
Used for: depletion
Treatment / Staining Regime: 100ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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F4/80 (macrophage)

Antibody against:F4/80 (macrophage)
Label: PE-Cy5
Clone: 145-2C11
Isotype:
Type: commercial
Source: BD Pharmingen cat# 553065
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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F4/80 pan

Antibody against:F4/80 pan
Label: PE
Clone: 145-2C11
Isotype: hamster IgG
Type: commercial
Source: BD Pharmingen cat# 553064
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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FoxP3

Antibody against:FoxP3
Label: PE
Clone: RM4-5
Isotype: rat IgG
Type: commercial
Source: BD Pharmingen cat # 553049
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.5ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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FoxP3 (rat too)

Antibody against:FoxP3 (rat too)
Label: FITC
Clone: GK1.5
Isotype:
Type: commercial
Source: BD Pharmingen cat# 553729
Restrictions on Use:
Used for:
Treatment / Staining Regime:
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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gamma delta TCR

Antibody against:gamma delta TCR
Label: PE-Cy5
Clone: GK1.5
Isotype: Rat IgG2a
Type: commercial
Source: Biolegend cat# 100410
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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H60

Antibody against:H60
Label: APC
Clone: RM4-5
Isotype: Rat IgG2b
Type: commercial
Source: Biolegend cat# 100515
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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Hamster IgG

Antibody against:Hamster IgG
Type: Isotype Control
Label: HTK888
Clone: commercial
Isotype: PE
Type: BioLegend 400907
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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Hamster IgG1, k

Antibody against:Hamster IgG1, k
Type: Isotype Control
Label: A19-3
Clone: commercial
Isotype: FITC
Type: Pharmingen 553971
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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Hamster IgG2, k

Antibody against:Hamster IgG2, k
Type: Isotype Control
Label: B81-3
Clone: commercial
Isotype: Biotin
Type: Pharmingen 550084
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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I-Ad

Antibody against:I-Ad
Label: purified
Clone: GK1.5
Isotype: rat IgG2a
Type: UCSF MAB core
Source: UCSF
Restrictions on Use:
Used for: depletion
Treatment / Staining Regime: 300 ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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IFNg

Antibody against:IFNg
Label: FITC
Clone: 30-F11
Isotype:
Type: commercial
Source: BD Pharmingen cat # 553079
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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IFNg

Antibody against:IFNg
Label: APC
Clone: DX5
Isotype: Rat IgM kappa
Type: commercial
Source: eBioscience Cat# 17-5971
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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IFNgRa

Antibody against:IFNgRa
Label: PE
Clone: 53-6.7
Isotype:
Type: commercial
Source: BD Pharmingen cat# 553033
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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IgG1 (isotype for CD8b)

Antibody against:IgG1 (isotype for CD8b)
Label: PE-Cy5
Clone: 53-6.7
Isotype: rat IgG
Type: commercial
Source: BD Pharmingen cat# 553034
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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IL-10

Antibody against:IL-10
Label: PE-Cy7
Clone: 53-6.7
Isotype: rat IgG
Type: commercial
Source: BD Pharmingen cat# 552877
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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IL-10

Antibody against:IL-10
Label: FITC
Clone: 53-6.7
Isotype: rat IgG
Type: commercial
Source: BD Pharmingen cat# 553031
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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IL-10R

Antibody against:IL-10R
Label: Fluor 488
Clone: FJK-16S
Isotype: Rat IgG2a
Type: commercial
Source: eBioScience # 53-5773
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.5ug
Performance: okay
Information provided by the lab of: Nora Disis

Reference:

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IL-12

Antibody against:IL-12
Label: FITC
Clone: GL3
Isotype: hamster
Type: commercial
Source: BD Pharmingen cat# 553177
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2 ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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langerin/CD207

Antibody against:langerin/CD207
Label: FITC
Clone: 39-10-8
Isotype: Mouse IgG3
Type: commercial
Source: BD Pharmingen cat# 553610
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.5ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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langerin/CD207

Antibody against:langerin/CD207
Label: PE
Clone: XMG1.2
Isotype: Rat IgG1
Type: commercial
Source: BD Pharmingen cat# 554412
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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Ly-6G (Gr-1)

Antibody against:Ly-6G (Gr-1)
Label: PE
Clone: JES5-16E3
Isotype: rat IgG2b
Type: commercial
Source: eBioscience Cat# 12-7101
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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Ly-6G (Gr-1)

Antibody against:Ly-6G (Gr-1)
Label: PE
Clone: C17.8
Isotype: rat IgG2a
Type: commercial
Source: eBioscience Cat# 12-7123
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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Ly6C

Antibody against:Ly6C
Label: FITC
Clone: RB-6-8C5
Isotype: Rat IgG2b
Type: commercial
Source: eBioScience # 11-5931
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.5ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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Ly6C

Antibody against:Ly6C
Label: purified
Clone: RB-6-8C5
Isotype: Rat IgG2b
Type: UCSF MAB core
Source: UCSF
Restrictions on Use:
Used for: depletion
Treatment / Staining Regime: 100ug
Performance: in evaluation
Information provided by the lab of: Nora Disis

Reference:

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MHC class II

Antibody against:MHC class II
Label: PE
Clone: 5D10
Isotype: Hamster IgG
Type: commercial
Source: eBioscience Cat# 12-5863
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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Mouse IgG1, k

Antibody against:Mouse IgG1, k
Type: Isotype Control
Label: MOPC-21
Clone: commercial
Isotype: FITC
Type: BioLegend 400108
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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Mouse IgG1, k

Antibody against:Mouse IgG1, k
Type: Isotype Control
Label: ?
Clone: commercial
Isotype: PE
Type: Pharmingen 03005A
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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Mouse IgG2b, k

Antibody against:Mouse IgG2b, k
Type: Isotype Control
Label: MPC-11
Clone: commercial
Isotype: PE
Type: BioLegend 400312
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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Mult1 (rat too)

Antibody against:Mult1 (rat too)
Label: PE
Clone: PK136
Isotype: mouse IgG2a
Type: commercial
Source: eBioscience Cat# 12-5941
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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NK1.1

Antibody against:NK1.1
Label: PE
Clone: A10
Isotype: Hamster IgG
Type: commercial
Source: eBioscience Cat# 12-5872
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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NK1.1

Antibody against:NK1.1
Label: PE
Clone: RD-41
Isotype: mose IgG1
Type: commercial
Source: eBioscience Cat# 12-5756
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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NK1.1

Antibody against:NK1.1
Label: PE
Clone: PK136
Isotype:
Type: commercial
Source: BD Pharmingen cat# 557391
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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NKG2D

Antibody against:NKG2D
Label: PE
Clone: CX1
Isotype: rat IgG2b
Type: commercial
Source: eBioscience Cat# 12-5881
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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PD-1

Antibody against:PD-1
Label: PE
Clone: MP6-XT22
Isotype: rat IgG1
Type: commercial
Source: eBioscience Cat# 12-7321
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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PD-L1

Antibody against:PD-L1
Label: purified
Clone: T2.5
Isotype: mouse IgG1
Type: commercial
Source: eBioscience Cat# 14-9024-82
Restrictions on Use:
Used for: blocking
Treatment / Staining Regime: 10ug/ml
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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PD-L1

Antibody against:PD-L1
Label: purified
Clone: PK136
Isotype: mouse IgG2a
Type: UCSF MAB core
Source: UCSF
Restrictions on Use:
Used for: depletion
Treatment / Staining Regime: 100 ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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PD-L2

Antibody against:PD-L2
Label: PE
Clone: 6C2
Isotype: rat IgG2b
Type: commercial
Source: eBioscience Cat# 12-9021-80
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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PDCA-1

Antibody against:PDCA-1
Label: Alex488
Clone: T2.5
Isotype: mouse IgG1
Type: commercial
Source: eBioscience Cat# 53-9024-80
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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Rae1 delta

Antibody against:Rae1 delta
Label: purified
Clone: MTS510
Isotype: rat IgG2a
Type: commercial
Source: eBioscience Cat# 14-9924-82
Restrictions on Use:
Used for: blocking
Treatment / Staining Regime: 10ug/ml
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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Rae1 gamma

Antibody against:Rae1 gamma
Label: purified
Clone: 1D11
Isotype: mouse IgG1
Type: commercial
Source: R&D Cat# MAB1835
Restrictions on Use:
Used for: in vivo blocking
Treatment / Staining Regime: 100ug ip
Performance: not determined
Information provided by the lab of: Nora Disis

Reference:

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Rat IgG1, k

Antibody against:Rat IgG1, k
Type: Isotype Control
Label: R3-34
Clone: commercial
Isotype: PE
Type: Pharmingen 553925
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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Rat IgG1, k

Antibody against:Rat IgG1, k
Type: Isotype Control
Label: RTK2071
Clone: commercial
Isotype: PE
Type: BioLegend 400407
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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Rat IgG1, k

Antibody against:Rat IgG1, k
Type: Isotype Control
Label: RTK2071
Clone: commercial
Isotype: Biotin
Type: BioLegend 400403
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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Rat IgG2a, k

Antibody against:Rat IgG2a, k
Type: Isotype Control
Label: RTK2758
Clone: commercial
Isotype: PE
Type: BioLegend 400508
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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Rat IgG2a, k

Antibody against:Rat IgG2a, k
Type: Isotype Control
Label: ?
Clone: commercial
Isotype: PE
Type: Pharmingen 11025A
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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Rat IgG2a, k

Antibody against:Rat IgG2a, k
Type: Isotype Control
Label: R35-95
Clone: commercial
Isotype: FITC
Type: Pharmingen 11024C, new = 553929
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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Rat IgG2a, k

Antibody against:Rat IgG2a, k
Type: Isotype Control
Label: RTK2758
Clone: commercial
Isotype: FITC
Type: BioLegend 400506
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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Rat IgG2b, k

Antibody against:Rat IgG2b, k
Type: Isotype Control
Label: RTK4530
Clone: commercial
Isotype: PE
Type: BioLegend 400608
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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Rat IgG2b, k

Antibody against:Rat IgG2b, k
Type: Isotype Control
Label: A95-1
Clone: commercial
Isotype: Biotin
Type: Pharmingen 553987
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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Rat IgG2b, k

Antibody against:Rat IgG2b, k
Type: Isotype Control
Label: RTK4530
Clone: commercial
Isotype: FITC
Type: BioLegend 400605
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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Rat IgM, k

Antibody against:Rat IgM, k
Type: Isotype Control
Label: RTK2118
Clone: commercial
Isotype: PE
Type: BioLegend 400807
Source:
Used for:
Treatment / Staining Regime:
Performance:
Information provided by the lab of: Robert Schreiber

Reference:

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TCR-b

Antibody against:TCR-b
Label: purified
Clone: JES052A5
Isotype: rat IgG1
Type: commercial
Source: R&D Cat# MAB417
Restrictions on Use:
Used for: in vivo blocking
Treatment / Staining Regime: 100ug ip
Performance: not determined
Information provided by the lab of: Nora Disis

Reference:

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TGFb

Antibody against:TGFb
Label: PE
Clone: 205326
Isotype: rat IgG2a
Type: commercial
Source: R&D Cat# FAB1155P
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2 ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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TLR2

Antibody against:TLR2
Label: PE-Cy7
Clone: HL3
Isotype: Hamster IgG
Type: commercial
Source: BD Pharmingen cat# 558079
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2 ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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TLR2

Antibody against:TLR2
Label: purified
Clone: 2.34
Isotype: mouse IgG
Type: UCSF MAB core
Source: UCSF
Restrictions on Use:
Used for: depletion
Treatment / Staining Regime: 100 ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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TLR2

Antibody against:TLR2
Label: purified
Clone: Cy1.2.4
Isotype: mouse IgG
Type: UCSF MAB core
Source: UCSF
Restrictions on Use:
Used for: depletion
Treatment / Staining Regime: 100 ug i.p.
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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TLR4

Antibody against:TLR4
Label: FITC
Clone: AL-21
Isotype:
Type: commercial
Source: BD Pharmingen cat# 553104
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2 ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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TNFa

Antibody against:TNFa
Label: PE
Clone: 1D4B
Isotype: rat IgG2a
Type: commercial
Source: eBioscience Cat# 12-1071-81
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.5ug
Performance: good
Information provided by the lab of: Nora Disis

Reference:

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Vb12 TCR

Antibody against:Vb12 TCR
Label: PE
Clone: M1/70
Isotype: Rat IgG2b
Type: commercial/purified
Source: BD Pharmingen cat#553311
Restrictions on Use:
Used for: FACS
Treatment / Staining Regime: 0.2ug
Performance: 1 log shift over iso
Information provided by the lab of: Ian Frazer

Reference:

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