Slide 009

Slide ID:


Collection of murine pulmonary tumors - Slide 009

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Submitting Investigator:

Anton Berns/Ralph Meuwissen

Mouse Age:

12 months

Mouse Sex:


Mouse Strain:



p53flox/flox Rbflox/flox

Genetic Modification Details:

Homozygous conditional knockout alleles for both p53 and Rb. Both genes become full null alleles upon Cre-mediated recombination.

Other Factors:

Mouse has been intratracheally infected with AdCre and sacrificed at 30 weeks post infection.

Gross Morphology:

Lung did contain macroscopic tumor lesions.

Method of Fixation:



Neuroendocrine carcinoma.
There is a densely cellular closely arranged neoplastic cell population that effaces the normal pulmonary architecture. The neoplastic population is arranged in a solid patternwith poorly defined nests and streams. The neoplastic cells are small to medium size and round with scant cytoplasm, finely granular chromatin and small nucleoli. The neoplastic cells are supported by a fine fibrovascular stroma. Adjacent compressed alveolar spaces contain small aggregates of neoplastic cells and swollen macrophages. Small bronchioles are lined by prominent hyperplastic robust epithelium and the lumen contain fibrin mixed with small neoplastic aggregates. There are occasional aggregates that resemble blood vessels filled with neoplastic cells and mixed with erythrocytes. There are small areas of necrosis scattered throughout the tumor. According to immunohistochemistry, the tumor cells contain variable amounts of synaptophysin and NSE, but not CD3 and CD45R antigens.


Meuwissen, R., Linn, S. C., Linnoila, R. I., Zevenhoven, J., Mooi, W. J., and Berns, A. Induction of small cell lung cancer by somatic inactivation of both Trp53 and Rb1 in a conditional mouse model. Cancer Cell, 4: 181-189, 2003.